// Rolling dF/F Normalization // Set averaging window window = 1 // ======================================= stk = getTitle(); run("Duplicate...", "duplicate"); mean = getTitle(); // Make rolling window stack run("Mean 3D...", "x=0 y=0 z=&window"); // Calculate difference imageCalculator("Subtract create 32-bit stack", stk, mean); diff = getTitle(); // Normalize imageCalculator("Divide create 32-bit stack", diff, mean); dFF = getTitle(); setMinAndMax(-1, 1); close(diff); close(mean);