// Select AvgIP image avgip = getTitle(); run("Duplicate...", " "); wrkimg = getTitle(); run("Median...", "radius=2"); run("Subtract Background...", "rolling=50"); run("Gaussian Blur...", "sigma=3"); setAutoThreshold("Huang dark"); setOption("BlackBackground", false); run("Convert to Mask"); run("Analyze Particles...", "size=100-Infinity clear add"); run("Set Measurements...", "mean redirect=None decimal=3"); // Use the ROI manager to select multimeasure. Save ROIs and traces. //roiManager("Multi Measure");