{"channelImages":{"list":[{"importKeyword":"_REF","name":"RFP"},{"importKeyword":"","name":"YFP"}]},"template":{"transformations":{"list":[{"pluginName":"RemoveStripesSignalExclusion","params":[[0],{"pluginName":"BackgroundThresholder","params":[2.5,3.0,3],"activated":true},"false"],"activated":true,"inputChannel":[0]},{"pluginName":"RemoveStripesSignalExclusion","params":[[0],{"pluginName":"BackgroundThresholder","params":[2.5,3.0,3],"activated":true},"true"],"activated":true,"inputChannel":[1]},{"pluginName":"SaturateHistogramHyperfluoBacteria","params":[{"pluginName":"BackgroundThresholder","params":[3.0,6.0,3],"activated":true},{"pluginName":"IJAutoThresholder","params":["Otsu"],"activated":true},0.45,10000],"activated":true,"inputChannel":[0]},{"pluginName":"AutoRotationXY","params":["Fluo Microchannel",-10.0,10.0,0.5,0.05,"BSPLINE5",10,{"list":[]}],"activated":true,"inputChannel":[0]},{"outputChannel":[1],"pluginName":"SimpleTranslation","params":[1.0,1.0,0.0,"NEAREST"],"activated":true,"inputChannel":[1]},{"pluginName":"Flip","params":["Y"],"activated":true,"inputChannel":[0]},{"pluginName":"CropMicroChannelFluo2D","params":[330,45,0,200,{"pluginName":"BackgroundThresholder","params":[3.0,6.0,3],"activated":true},0.5,0,0,0,0,10],"activated":true,"inputChannel":[0]},{"pluginName":"ImageStabilizerXY","params":[1000,1.0E-8,"1",20,{"pluginName":"CropMicroChannelFluo2D","params":[330,45,0,200,{"pluginName":"BackgroundThresholder","params":[3.0,6.0,3],"activated":true},0.5,0,0,0,0,10],"activated":true},{"list":[[[1],1.0,-1.0,0]]},"false"],"activated":false,"inputChannel":[0]}]},"trimFramesEnd":0,"frameDuration":120.0,"trimFramesStart":0,"useImageScale":"Use Image Calibration","scaleXY":1,"scaleZ":1},"imagePath":[],"outputPath":[null],"structures":{"list":[{"name":"Microchannel","channelImage":[0],"manualSegmenter":{"pluginName":"no selection","activated":true},"segmentationParent":[-1],"brightObject":"Bright object over dark background","parentStructure":[-1],"allowSplit":"no","allowMerge":"no","objectSplitter":{"pluginName":"no selection","activated":true},"processingScheme":{"pluginName":"SegmentAndTrack","params":[{"pluginName":"MicrochannelTracker","params":[{"pluginName":"MicroChannelFluo2D","params":[350,40,20,{"pluginName":"IJAutoThresholder","params":["Otsu"],"activated":true},0.3,200],"activated":true},40,0.5,0.05,100],"activated":true},{"list":[]},{"list":[]},{"list":[{"pluginName":"TrackLengthFilter","params":[100,0],"activated":true},{"pluginName":"RemoveTracksStartingAfterFrame","params":[0],"activated":true}]}],"activated":true},"manualObjectStrechThreshold":0.5},{"name":"Bacteria","channelImage":[0],"manualSegmenter":{"pluginName":"no selection","activated":true},"segmentationParent":[0],"brightObject":"Bright object over dark background","parentStructure":[0],"allowSplit":"yes","allowMerge":"no","objectSplitter":{"pluginName":"no selection","activated":true},"processingScheme":{"pluginName":"SegmentAndTrack","params":[{"pluginName":"BacteriaClosedMicrochannelTrackerLocalCorrections","params":[{"pluginName":"BacteriaFluo","params":[0.12,100,10,3,40,4,1,2,0,0.2],"activated":true},0.85,1.5,0.1,0.5,0.45,{"action":"From Segmenter","params":{"Adaptative By Frame and Y":[0.06,1,10,30],"Adaptative By Frame":[0.06,1,10]}}],"activated":true},{"list":[]},{"list":[]},{"list":[]}],"activated":true},"manualObjectStrechThreshold":0.5},{"name":"Mutation","channelImage":[1],"manualSegmenter":{"pluginName":"no selection","activated":true},"segmentationParent":[1],"brightObject":"Bright object over dark background","parentStructure":[0],"allowSplit":"no","allowMerge":"no","objectSplitter":{"pluginName":"no selection","activated":true},"processingScheme":{"pluginName":"SegmentAndTrack","params":[{"pluginName":"LAPTracker","params":[{"pluginName":"MutationSegmenter","params":[2.0,5,0.9,0.75,0.9],"activated":true},[1],0.8,0.8,3,0.15,0.82,2.0,[8.0,14.0]],"activated":true},{"list":[{"pluginName":"BandPass","params":[0.0,8.0,"None",5.0],"activated":true}]},{"list":[{"pluginName":"FeatureFilter","params":[{"pluginName":"Quality","activated":true},0.8,"true","true"],"activated":true}]},{"list":[]}],"activated":true},"manualObjectStrechThreshold":0.5}]},"importMethod":"One File Per Channel And Position","positions":{"list":[]},"measurements":{"list":[{"pluginName":"BacteriaLineageMeasurements","params":[[1],"BacteriaLineage"],"activated":true},{"pluginName":"ObjectFeatures","params":[[1],{"list":[{"pluginName":"Size","addParams":["Size"],"params":["Unit"],"activated":true},{"pluginName":"FeretMax","addParams":["Length"],"params":["Unit"],"activated":true},{"pluginName":"Mean","addParams":["MeanIntensity"],"params":[[1]],"activated":true}]}],"activated":true},{"pluginName":"MutationTrackMeasurements","params":[[1],[2]],"activated":true},{"pluginName":"ObjectInclusionCount","params":[[1],[2],10.0,"false","MutationNumber"],"activated":true},{"pluginName":"ObjectFeatures","params":[[2],{"list":[{"pluginName":"Quality","addParams":["Quality"],"activated":true}]}],"activated":true},{"pluginName":"RelativePosition","params":[[1],[0],"Geometrical","Corner","RelativeCoord"],"activated":true}]}}