ThunderSTORM provides a variety of post-processing methods and tools which can be applied to the results obtained from previously processed SMLM data. The software includes methods for correcting the data by removing molecules with, e.g., poor localization accuracy or small local density, for merging molecules present in subsequent images, and for position correction of molecules caused by lateral drift of the sample and/or by axial stage scanning. Users can also define the order in which the different post-processing steps are applied. Localization results can be imported/exported to/from ThunderSTORM in a variety of data formats which allows full compatibility with other SMLM localization software.
A list of coordinates with sub-pixel positions of the molecules.
A corrected list of coordinates with sub-pixel positions of the molecules.
The order of post-processing steps is user-specified. However, we recommend the following order: Remove duplicates (if MFA was enabled), filtering (to remove outliers), Z-stage offset (if applicable), drift correction, and finally merging.