Difference of averaging filters

The difference of averaging filters [1] is another type of a band-pass filter. The filtered image is obtained by subtracting images filtered with two averaging filters having different user-specified kernel sizes,

F=I*K_{\mathrm{AV1}}-I*K_{\mathrm{AV2}}\,.

Similar to the difference-of-Gaussians filter, the size of the kernel K_{\mathrm{AV2}} has to be larger than K_{\mathrm{AV1}}.

Threshold for approximate localization of molecules

The threshold value can be specified by users as an expression combining mathematical functions and operators with variables based on the current raw or filtered image. Variables provided by this filter are:

DoB.I current raw input image
DoB.F corresponding filtered image
DoB.B1 result of filtering only with K_{\mathrm{AV1}}
DoB.B2 result of filtering only with K_{\mathrm{AV2}}

References

  • [1] F. Huang, S. L. Schwartz, J. M. Byars and K. A. Lidke(2011) Simultaneous multiple-emitter fitting for single molecule super-resolution imaging, Biomedical Optics Express 2 (5), pp. 1377–93. External Links: Document. Cited by: Difference of averaging filters.