Finding approximate positions of molecules

Finding approximate positions of the molecules in the input images follows the image filtering and feature enhancement step. ThunderSTORM offers three algorithms for this purpose: detection of local intensity maxima, non-maximum suppression, or calculation of the centroid of connected components of segmented objects. All three methods need a user-specified threshold defining an intensity below which possible molecular detections will be rejected.

• Input:

  A filtered image of size pixels.

• Output:

  A list of coordinates with approximate molecular positions.