ThunderSTORM provides two models to describe the relationship between the axial position of a molecule and its imaged size. The first model is represented by a pair of second degree polynomials, the second model is introduced by Huang et al. [1].
Polynomial defocusing model
Huang’s defocusing model
Rotated elliptical Gaussian function PSF model (3D using astigmatism)
Calibration of the imaging system