input:
Image directory:
directory where the images are read from,
all files ending on ".tif" are considered;
please refer to the webpage for
further information on how the file names should be formatted
Mask directory:
directory where the segmentation information for
the images is read from; the directory can be identical to the
image directory
Mask format:
expected format of the segmentation data files
Output and working directory:
directory for intermediate and
final results
Calculate features?
by disabeling this option the time-consuming
feature calculation can be omitted, however, in this case the feature
directory is expected to already contain the feature files
Feature directory:
directory where the calculated features are
saved to and from where they are read if feature calculations are omitted
Number of feature clusters:
number of clusters to be used for
feature vector clustering, should approximately refer to the expected number
of structural patterns appearing in the cells
Do PCA in stage II?
enable or disable the PCA prior to the
pairwise distance calculations in stage II of the approach
output:
Resulting chart plots for each group:
cluster distributions for each cell categorized into cell groups
Resulting box-whisker plot:
box plot of the relative frequecies
of appearance of each cluster for the different cell groups
For more details about the operator and additional information on the parameters refer to its webpage: http://www2.informatik.uni-halle.de/agprbio/mitobo/index.php/Applications/ActinAnalyzer2D.